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KMID : 1145520170030020054
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Journal of Radiopharmaceuticals and Molecular Probes
2017 Volume.3 No. 2 p.54 ~ p.58
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Simple measurement the chelator number of antibody conjugates by MALDI-TOF MS
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Shin Eun-Bi
Lee Ji-Woong
Lee Kyo-Chul
Shim Jae-Hoon
Cha Sang-Won
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Abstract
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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS) is one of the powerful methods that enable analysis of small molecules as well as large molecules up to about 500,000 Da without severe fragmentation. MALDI-TOF MS, thus, has been a very useful an analytical tool for the confirmation of synthetic molecules, probing PTMs, and identifying structures of a given protein. In recent nuclear medicine, MALDI-TOF MS liner ion mode helps researcher calculate the average number of chelator(or linkage) per an antibody conjugate, such as DOTA-(or DFO-) trastuzumab for labeling a medical radioisotope. This simple technique can be utilized to improve the labeling method and control the quality at the development of antibody-based radiopharmaceuticals, which is very effected to diagnosis and therapy for in vivo tumor cells, with radioisotopes like 89Zr, 64Cu, and 177Lu. To minimize the error, MALDI-TOF MS measurement is repeatedly performed for each sample in this study, and external calibration is carried out after data collection.
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KEYWORD
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MALDI-TOF MS, chelator number, trastuzumab, antibody conjugate, radioisotope
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